628nm CWL, 50mm Dia, 32nm Bandwidth, OD 6 Fluorescence Filter

Product Details Common Wavelengths for Popular Fluorophores Excitation Filters and Emission Filters Available >93% Transmission >OD 6 Blocking, <3% from Edge of Bandwidth Fluorescence Filter Sets and Pre-Mounted Fluorescence Filter Cube Sets Also Available Our TECHSPEC Fluorescence Bandpass Filters perfectly match common fluorophores' absorption and emission characteristics in fluorescence imaging applications. Excitation and emission filters are available, featuring high transmissions in the passband (≥ 93% for VIS designs) and >OD 6 blocking outside of the passband. These filters are also ideal components in applications using spectroscopic fluorescence detection, including analytical chemistry and biomedical instruments such as DNA sequencers and polymerase chain reaction (qPCR) devices. Each filter features state-of-the-art hard-sputtered coatings and is mounted in a black anodized ring for easy system integration. Fluorescence microscopy and spectroscopy applications typically use a combination of three filters for each color channel (detection of individual fluorophores), an excitation and an emission bandpass filter, as well as a dichroic filter (also dichroic beamsplitters, such as our Fluorescence Dichroic Filters and High-Performance Fluorescence Dichroic Filters ). More information on fluorescence microscopy and an example beam path can be found in the AppNote Fluorophores and Optical Filters for Fluorescence Microscopy . In short, excitation filters “clean up” the excitation light used to excite the fluorophores in the sample. On the other hand, emission filters allow only the emitted fluorescence to pass through to the detector while effectively blocking the excitation light and different wavelengths, producing high-contrast images and superior image clarity. Our fluorescence bandpass filters are engineered with cutting-edge technology and are designed to enhance signal-to-noise ratios, improve contrast, and deliver excellent performance in optical imaging applications. These filters are designed to be widely compatible with microscopes from Nikon, Olympus, and Zeiss, making them suitable for various imaging applications, including widefield, confocal, multiphoton, and other fluorescence microscopy modalities. Whether you are imaging fixed cells, live cells, or tissues, our filters are engineered to deliver consistent and reliable image quality. Additionally, these filters can be used for fluorescence detection in qPCR, flow cytometry, or multi-well plate readers in applications such as immunofluorescence or high-throughput screening in which fluorescent molecules or dyes detect specific molecular structures or DNA sequences. TECHSPEC Fluorescence Bandpass Filters are optimized for maximum transmission within a specific wavelength range. They effectively isolate fluorescence signals from background noise, enhancing sensitivity and enabling more accurate detection of fluorophores. Explore our range of filters today and unleash the full potential of your fluorescence microscopy setup. Specifications General Type: Bandpass Filter Compatible Fluorophore: SpectrumRed Emission / Cy5 Excitation Physical & Mechanical Properties Diameter (mm): 50.00 +0.00/-0.10 Clear Aperture CA (mm): 45.21 Construction: Mounted in Black Anodized Ring Physical Durability: Adhesion per MIL-PRF-13830B, Section C.4.5.12 Moderate abrasion per MIL-PRF-13830B, Section C.4.5.11 Cleaning per MIL-C-48497A Section 4.5.4.2 Substrate Thickness (mm): 2.00 ±0.25 Optical Properties Angle of Incidence (°): 0 ±5 Bandwidth (nm): 32.00 OD 6 Blocking Wavelength Range (nm): 250 – 598 & 659 – 925 Optical Density OD (Average): ≥6.0 Average Transmission (%): >93 over Bandwidth Center Wavelength CWL (nm): 628.00 Full Width-Half Max FWHM (nm): 38.00 Substrate: Many glass manufacturers offer the same material characteristics under different trade names. Learn More Fused Silica (Corning 7980) Coating: Hard Coated Surface Quality: 60-40 Transmission (%): 93.00 Blocking Wavelength Range (nm): 250 – 1100 Transmitted Wavefront, RMS: λ/4 (prior to coating) Threading & Mounting Mount Thickness (mm): 3.5 ±0.1 Environmental & Durability Factors Environmental Durability: Humidity per MIL-STD-810H, Section 507.6 Temperature per MIL-STD-810H, Section 501.7 and 502.7 Regulatory Compliance RoHS 2015: Compliant × Certificate of Conformance: View × Reach 247: Compliant ×